Factual error: In the scene when they are leaving the Pittsburgh Zoo, the sign indicates to turn right for Interstate 79. However Intestate 79 is about 15 miles to the west of the zoo. Also, it is suggested that Interstate 79 is the turnpike (a tolled road), when actually Interstate 76 is the turnpike and Interstate 79 is not tolled.
Plot hole: During the scene where John (Russell) decides he needs to break into the medical van, he watches an infamous YouTube video about using a tennis ball to open "car door locks." Unfortunately for John, the YouTube video was faked, and in reality was proven to be fake on the TV show MythBusters.
Revealing mistake: At the end of the film when the two Detectives are checking the sewer for the missing button. As the male Detective is lifting the sewer, the inner sewer lid paint peels back, exposing a white color of the sewer lid. Which makes you realize the sewer is likely made of a light wood or plastic (making it easier for actors to lift lid). It is obvious the "Props Crew" didn't do a good job in painting the sewer. It is made more obvious when the female Detective tries to hide the peeling paint by very quickly sliding her hand over the exact spot its peeling. Then in the next shot the male Detective does the same, but in the corner.
Continuity mistake: The silver SUV in which John, Laura and their son are escaping, changes several times from one to two inspection decals on its windshield. Firstly there are two decals reading 9_10. Right after Laura tries to step out of the running car and they almost collide with a truck, the SUV comes to a full stop and there is a single decal reading 5_10. When they are at the checkpoint leaving Pennsylvania we go again to two decals. Additionally, when two decals are present, they sometimes read 9_10 and some other times 11_10.