Continuity mistake: The actor who plays Ben as a child has brown eyes, but Nicholas Cage has blue eyes.
Factual error: In the scene when Ben, Abigail and Riley are on the tower of Independence Hall, they look at the shadow marking the secret place where the glasses are hidden. It is 3:20. The shadow of the cross is in the second third of the pillar. When Ian is on the tower the shadow has already moved to the top of the wall. As the others have left Independence Hall at 3:28 the shadow must have traveled the distance in about 4 minutes. This is far too quickly. (01:12:20)
Continuity mistake: In the treasure vault Riley stands in front of that emerald statue, and on its chest you can see a part which is not covered in dust. In the next shot the whole chest is covered in dust. The clean area coincides with the later placement of Riley's cheek, which means the first shot was actually taken after the embracing and implemented before. (01:50:45)
Revealing mistake: When Ben and Ian get the two different fingerprints from two different people, Mr. Ferguson [random security guard] and Abigail Chase, they are the exact same fingerprints. This can be confirmed on the DVD with pause and zoom. Moreover, the two scans show exactly the same emphasis/pressure on different regions within the print, with more light and detail on the left side and little in the center. (00:39:15)
Other mistake: When Gates writes down the numbers from the back of the Declaration of Independence he forgets one number. On the document there are the following numbers: 11-6-6, 12-18-7, 14-3-7. Ben however only writes down 11-6-6, 14-3-7 and then keeps on. (01:01:10)
Continuity mistake: When Gates and Dr. Chase are in the store to buy new clothes, Abigail is coming out of the changing cubicle. She opens the door wing while having both hands on the edge, when the shot changes, she leans on the edge with both forearms. (01:07:50)
Continuity mistake: When Ben finds the glasses at Independence Hall, it is 3:20 because of daytime savings time. However when the bad guys chase Abigail and Riley in the market they pass a large clock showing the time to be 2:37. (01:12:15 - 01:17:10)
Suggested correction: Actually, many places at a market like that sell old antiques and even old clocks. That could simply be one of them. Even if it was supposed to tell time, people often forget to set their clocks forward or back for Daylight Savings Time, and that could have happened here. Many of the clocks at my home and school are currently like that.