Visible crew/equipment: During the briefing Weyland says "But it's not on the ice, it's two thousand feet below it" it cuts to a shot behind Maxwell with an overview shot of the crew. If you look behind Maxwell you can see the tip of a head moving into picture. In the previous shot we could see no one was standing behind Maxwell, and it couldn't be Sebastian cause he went back to his seat.
Continuity mistake: When Lex and the predator are going up the tunnel at the near the end of the movie. Lex has the alien head shield on her left arm. But when they are speeding up the tunnel, the shield is not on either arm. If you pause it, you can see the shield missing. When they emerge out of the tunnel it is back on her arm.
Continuity mistake: When the group are in the pyramid, just after they have discovered the weapons inside the sarcophagus, they begin to take them out. When Sebastian says, "Don't touch them", all the doors start to close, and Lex runs past a guy twice. (Slo-mo may be necessary.)
Factual error: The expedition leader claims that Bouvet Island is not under any national control, because of the Antarctic Treaty. However, because of its remoteness from the continent of Antarctica, Bouvet Island is actually not included in this treaty and Norway has full ownership of this island.
Continuity mistake: When Lex Miller and Sebastian are talking at the side of the ship at the beginning, Miller says "Ii wish you'd consider coming with us ", it cuts to a shot from the side/back, she is looking either in Sebastian's direction or out to sea, yet in the following shot she is looking at Miller's face.
Continuity mistake: Close to the beginning, when the group is inside the pyramid below the sacrificial chamber, when the shot is from behind Lex and the others, note where the green light is, directly in the middle of the stairs. When it cuts to an overhead view, it's more to the left than the right.