Continuity mistake: When the gorilla comes out of the cage and confronts Willa, she has a basket containing several bunches of bananas, all yellow. After the encounter, the gorilla reaches out and takes a bunch of the bananas, but they are green. (00:45:00 - 00:45:45)
Continuity mistake: When Rollo and Willa check the computer for evidence of Vince's thievery Willa takes some papers from the bookshelf with her right hand. When Rollo reaches back for the papers without turning around he inadvertently cups her breast. When the camera zooms wide the papers appear in Willa's left hand. (01:05:55)
Revealing mistake: Near the beginning of the movie, when all the zookeepers are being 'savaged' by bandicoots and chipmunks, a woman falls down and really hurts herself. Later in this scene, the woman is dropped from her stretcher, except before she is dropped, there is no-one holding on to the end of the stretcher nearest to us.
Audio problem: When Vince is yelling, "He's talking to himself, and I'm the loony?" the second shot has him saying something else, possibly the first part of the line again.
Plot hole: The animal keepers take some tame animals to Rollo's office carrying them in their arms. When they leave the animals behind so that Rollo can shoot them they put them into cages. They didn't bring any cages with them nor were any already in the office when they came in. (00:13:35)
Revealing mistake: When Vince tells Rod, 'I am not a wuss', Rollo, who's in the background, also mouths the line.