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Continuity mistake: While Captain Stacy is dying Spidey is right next to him with his left arm on him, but in the next shot he is now 1 or 2 feet away.

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Continuity mistake: While Peter is sleeping in the subway he has something on his head as a pillow which suddenly disappears.

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Continuity mistake: While uncle Ben and Peter talks with Gwen in the school hall, her hands and the folder change positions between shots.

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Continuity mistake: After the lizard attacks Spidey in the school he left his coat in the bathroom, but when he gets to the sewer he has it back again.

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Continuity mistake: Before the lizard assaults Gwen in the lab, she has her hands on the device with nothing else, but suddenly has the spray bottle and the lighter.

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