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Trivia: In the Turtle's trophy room, there is an Ooze canister on the shelf. In most incarnations of the TMNT, the company on this canister is labeled TCRI, but in the second live-action movie, it is TGRI. According to the director's commentary, they have the canister here split in just a way that you can't tell if it's a C or a G, helping to leave it ambiguous if this film is part of the 90s movie continuity, part of the 03 series, or its own continuity.

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Trivia: Famous voice actor, Mako, is the voice of Splinter in this film; however, he passed away in 2006. This movie was released the next year in 2007, making it one of two films released posthumously with Mako as a voice.

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Trivia: Mikey Kelley is the voice of Michelangelo in this movie, and he was the original voice actor for Ratchet in the first Ratchet and Clank game. James Arnold Taylor is the voice of Leonardo in this movie, who replaced Mikey Kelley as Ratchet in Ratchet and Clank 2: Going Commando, and onward. So both voice actors for Ratchet play two different Turtles in this film.

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