Trivia: The image/symbol of the Xenomorph on the floor of the sacrificial chamber is the same or similar to the symbol used in the marketing of Alien 3 and seen on the movie's cover and posters. This same symbol is also on the necklaces of the people being sacrificed, seen both in the flashbacks of them lying down, and sometimes visible on the decayed corpses in the modern time of the movie.(00:35:37)
Trivia: The Alien Queen in Aliens was not able to be built how originally intended due to the limitations at the time. However, with this film, Anderson was fully able to have created the full Alien Queen that James Cameron had originally envisioned—15 feet tall with an 8-foot head and 47 points of articulation. It was more sophisticated than the T-Rex from Jurassic Park and took 8 people controlling it.
Trivia: The helicopter approaches an ice breaker ship called the Piper Maru. This is named after the X-Files episode of the same name, which also had an ice breaker ship called the Piper Maru, which in that show was named after Gillian Anderson's daughter. Coincidentally, Lance Henriksen, who is a main character in this movie, also appeared in X-Files and got his own spin-off show as well, Millennium.(00:06:42)
Trivia: The camera lens and style at the start of the film is a clear reference to Hal 9000 from the film 2001: A Space Odyssey in how it's shaped and looks, as well as the framing and blocking with the film camera.(00:01:33)
Trivia: For the title screen, the title of the film Alien vs Predator is first spelled out in the Yautja (Predator race) language. Then it changes to the green text from Mother in the first Alien movie, as a way of having the title shown in a style from both franchises.(00:00:42)
Trivia: At one point, based on the comics, a spec script was written by Peter Briggs and was going to be directed by Guillermo del Toro. However, they both opted out of the film to go write and direct Hellboy. That original script was shelved, but not lost as it was repurposed and used to make the Alien vs Predator arcade game instead, with a different script then written for this film.
Stupidity: The lead Predator got infected with a Xeno chestburster, and at the end of the movie, his body is shown on the ship with it bursting out. This is what sets up the sequel. However, the Predators have been coming to Earth for centuries, and they have also been hunting the Xenos for centuries and know their behaviours and breeding habits. They have X-ray capability and even use it in this film to show a human is infected. Yet they never scan his body before bringing it on their ship. And yes, I know there's the whole thing about them coming out victorious, it seems, and the other Predators likely would assume they were untouched. That doesn't make it not stupid that they still didn't scan his body to be sure, as they know what these things are capable of. And of course, in the sequel, that stupidity bites them in the ass.