Corrected entry: After Amanda gets the key from the pit of syringes, she throws it up to Xavier, who frantically tries to put it in the lock, but drops it, and the time expires and the door locks. There was a syringe with a green liquid in it attached to the key, and, seeing as how all of the other presumed antidotes were green, that one probably was as well. So, why didn't anybody inject it? Out of all the people there, at least one of them should have noticed it, especially Xavier, since he was obviously the most anxious person to get an antidote, and he was the one who was handling it.
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Correction: Perhaps since it wasn't mentioned by Jigsaw, no one considered that idea. Character Mistake and not a movie mistake.
moviemogul