Continuity mistake: When the antimatter explodes in the helicopter, the the shock-wave blasts off part of the roof inside St. Peter's Basilica, above the alter, obliterating everything below. However, in the scene where the Camerlengo self immolates himself at the altar close to the end of the movie, there is no evidence of the shock wave earlier, the candles are burning, oil lamps are lit and alter is intact.
xx:xx:xxBernie Lu Huey
Continuity mistake: In the collider Dr. Vetra gains access to the antimatter storage area by resting her chin in the small metal "dish" that aligns her face with the optical scanner. When she removes her face there is a small blot of blood on the side of her chin from the scientist's eyeball that the assassin ripped out (to gain access earlier). However, when we see the metal dish there is a drop of blood on the side (that ends up on Vetra's face) AND a small puddle of blood in the chin rest that dripped from the avulsed eye. Despite Vetra resting her entire chin in the chinrest none of the blood from the puddle shows up on the front of her face.
Continuity mistake: When Landon and the Vatican Police arrive at the Piazza del Popolo at 7:59pm when looking for the first victim, the overhead shot shows shadows cast by their car and tourists in the area are at a very shallow angle (shadows 3 - 4 times the length of the height of objects casting them), indicating that it's late in the day. However, the shots from ground level show shadows cast at much steeper angles (only about 1/2 - 3/4 of the length of the object height), indicating that it's much closer to noon.
Continuity mistake: When the new threat is shown to Professor Langdon, the first time he asked it to be stopped, the threat says "We will brand your preferiti and sacrifi-" it end there. But when it was played again two minutes later, it repeated to "and sacrifice them to the altars of Science."