Continuity mistake: Near the end of the film Eric Stoltz stops Jon Voight from shooting Jennifer Lopez by stabbing him with a tranquiliser dart. Jon Voight tries to strangle him but the tranquiliser kicks in and stops him. They put him to bed and his throat wound has started bleeding heavily. When they get back to him after killing the last snake, there is no sign of blood anywhere on him.
Continuity mistake: When Ice Cube and J-Lo are fighting the final battle on the boardwalk, Ice Cube hits the snake in the head with an ax a number of times. When the shot shifts to the snake falling, the ax is embedded in its head, but when the shot shifts back to Ice Cube he is still holding the ax.
Continuity mistake: Right after Westridge is caught by the Anaconda and the tree comes crashing down, the boat is first right below it, next shot it is nowhere to be seen, even at a broad angle, and then back to being there again. (01:08:16)