Other mistake: In the scene where Tzekel-Kan reveals the big tiger thing, he is on his knees facing it. He says "do as I command" and raises his right arm and the tiger raises his left arm. The tiger and Tzekel-Kan should both raise the same hand because the tiger is built to mimic Tzekel-Kan's exact moves.
The Road to El Dorado (2000)
1 other mistake
Directed by: Bibo Bergeron, Will Finn, Don Paul, Jeffrey Katzenberg
Starring: Kenneth Branagh, Kevin Kline, Armand Assante, Rosie Perez
Continuity mistake: When Cortes comes to the El Dorado island, he sees that Miguel and Tulio's footprints lead directly from the boat to the jungle. But this is not what happened. Miguel and Tulio arrived to the island, bumped into two skeletons, ran back to the boat, walked around the beach for a while, and THEN went into the jungle. This is what the footprints should have shown.
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Trivia: Just before the Gods Speaker finds the picture of the jaguar in the book, you see a picture of someone fishing off a crescent moon: the Dreamworks logo.
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Answer: Miguel had just told Chief Tanni how loyal Tulio and he were to each other. Then he overheard Tulio saying to Chel that he wanted to go to Spain with her, ONLY with her "Forget Miguel." That's why Miguel was so upset, because Tulio excluded him from his plans and his life.