Continuity mistake: The government forces are rounding up the foreigners outside the bar. Bloodsport finishes his beer and puts it down; the bottle is facing opposite directions in the two shots, just look at the label. (01:02:10)
Continuity mistake: At the beginning of her escape, Harley faces three guards. The rightmost one is smoking a cigarette, then has no cigarette in his mouth, then he has it again. (01:11:25)
Continuity mistake: After killing the guards all around her, Harley rushes into the next room, kicking the door open, and begins fighting in close quarters with more. She breaks the arm of one of the guards who tries to stab her through the bars. In the sequence, the red wrap is covering her fingers in one shot, it's underneath them in the next. (01:12:15)
Continuity mistake: When Peacemaker sticks the detonator inside the explosive after the humorous argument with King Shark, the detonator is suddenly lower in the last shot. (01:21:45)
Continuity mistake: An personal assistant brings news about the attack on Jotunheim. Suarez turns towards her holding a sheet, but in the previous shot he already threw the document on the desk striking a pensive pose. (01:22:05)
Continuity mistake: Seeing all the Starro drones being held captive in the "Dirty Little Secrets" section, Rick Flag says "Good God". Thinker replies "If God existed..." etc. During this sentence Flag lowers the gun slightly before the first cut, but the gun is angled higher as it cuts to Peter Capaldi and even higher in the wider shot. (01:23:05)
Continuity mistake: When Peacemaker kills Rick Flag by stabbing him in the heart, the depth of the object changes between the close-up shot of Peacemaker's hand and the next shot, where we can see the part sticking out of Flag's chest has increased. This is before Peacemaker hits the object deeper. (01:31:05)
Continuity mistake: When Flag collapses after his fight with Peacemaker, his hand is off the shower tray. Peacemaker then talks to Ratcatcher 2, and Flag's hand is well on the tray. (01:31:40 - 01:32:05)
Continuity mistake: When Amanda Waller orders the blonde subordinate to "Open it", the digital clocks show the same times shown minutes earlier when they had yet to wake up, contact them, talk to them, order them to retreat, witness their change of mind. All of which certainly took longer than a minute. (01:46:45 - 01:49:35)
Continuity mistake: When the people in the control room wake up and find out there's "A freakin' Kaiju", the clocks are showing entirely different timezones from the previous instances, with a wild difference from one another (earlier they were a couple hours apart, now we have even 9 hours), and none of which matching the time when the action supposedly takes place (it was late morning, past 10 AM as shown by the surveillance room clock and the phone in the hands of Harley's torturer). (01:46:45)
Continuity mistake: After Waller issued her order to leave Corto Maltese to its fate, Bloodsport turns his back to Starro that is wrecking the city and walks towards the camera. Just a moment before the cut, Margo Robbie is shrugging, a gesture that is supposed to happen in the next shot, and she in fact repeats it in the next shot a second later. (01:48:30)
Continuity mistake: When Polka-Dot Man shoots his projectiles at his giant mom, he's standing in the sun or in the shade depending on the camera angle. (01:52:45)
Continuity mistake: When Polka-Dot Man hurts Starro's leg, its minions cry out in pain as well in unison, but they do it holding their knee or thigh in one shot, their ankle/calf in the next. (01:53:00)
Continuity mistake: When Harley and not-Milton join the others at the end of the movie, shadow areas appear and disappear between shots (rather obvious when King Shark asks his first "Num-num?"). (02:01:10)
Answer: Yes, it did.