Plot hole: When Ripley goes to access the computer Mother, the entry door opens and closes with a hissing noise, a couple of minutes later you see Ash next to Ripley with a smile on his face. How did Ash manage to open the same door without it making the hissing sound? Ash could not have been in the room before Ripley came in - once Ripley has sat down at the console, it rotates on a 90 degree angle, this would have made it impossible for Ash to be hiding behind a desk or a computer console without being seen by Ripley. There are many doors on the ship that make the same hissing sound when the doors open, an obvious example is the infirmary door. And Ash couldn't have disabled the pneumatics of the door, because when Ripley exits the room, the hissing noise is heard again. (01:18:40 - 01:20:00)
Plot hole: Supposedly the plague broke out the moment the rats poured into the docks, as well as the plague breaking out when the rats got on the boat. However, these are domesticated rats. Only the fleas on black rats would carry the "Black Death," which is spread by fleas that aren't carried by other rats.