Supernatural

Hell House - S1-E17

Revealing mistake: Dean gets his phone and snaps a picture of Sam when he's asleep. You see his phone take the picture but the screen is then white, showing he didn't really take anything.

Nightmare - S1-E14

Revealing mistake: When Max opens the bedroom door to confront his step-mother and Dean, using his telekinesis powers, you can see the equipment used to open the door, a rope pulling the door on the floor. (00:34:19)

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Skin - S1-E6

Revealing mistake: Sam and Dean do a U-turn out of the garage at the start and leave skid marks on the road. However, there are already skid marks from previous take in the exact spot they turn. (00:04:10)

Ssiscool

And Then There Were None - S6-E16

Revealing mistake: Samuel is very much dead, laid out on the table in the canning factory employee lounge, but as Sam stands over him, talking about whether or not it was right to gank him, you can see Samuel's chest rising and falling as he breathes. (00:26:30)

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Answer: Over the next few episodes, it is revealed that something is not quite right with Sam - that his soul is missing since he returned from Hell, and he does not feel any emotion or attachment to his brother.

Sierra1

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