Trivia: "Mad Dog" Tannen's actions after shooting Marty are a perfect homage by Thomas F Wilson to Lee Marvin in "The Man Who Shot Liberty Valance." He even gets the facial expressions, the mumbled and sarcastic thanks and the jaunty angle with which he holds the gun right.
Trivia: Dr. Curt Connors, Peter's one-armed teacher in the film, is the man who will later become The Lizard, one of Spider-Man's longest running enemies.
Trivia: Early on in the movie, Sam is watching Star Trek with Leonard Nimoy, who also plays Sentinel Prime. Also, Wheelie's comments on the episode are relevant to Sentinel Prime. (Just replace the word Spock with Sentinel Prime).
Trivia: Monica Bellucci's dress is some what see through. You can see that she isn't wearing underwear. (01:05:10)
Trivia: The first alien in the mail room that Will Smith starts to talk to in alien language is a rapper known in real life as Biz Markie. He is really making those noises with his mouth. (00:28:35)
Trivia: Steven Spielberg has a cameo as one of the doctors when Elliot and E.T. are 'hospitalized' inside the house.
Trivia: Some of the members of Captain America's wartime elite squad are taken from Marvel Comics WWII based "Sgt Nick Fury and the Howling Commandos" - such as Dum Dum Duggan and Gabe Jones.
Trivia: When composer Éric Serra showed Inva Mula, the Albanian soprano who was going to sing the Diva Dance, the sheet music for that part, apparently she laughed and said that it was impossible for the human voice to switch from high to low notes that quickly. She got her way: The part that ended up in the film is not a single piece of audio, rather it's several pieces stitched meticulously together.
Trivia: SPOILER: At the end on Nick Fury's gravestone, under his name is the passage from Ezekiel 25:17: "The path of the righteous man is beset..." This was the start of a big monologue made famous by Samuel L Jackson in Pulp Fiction. (01:59:25)
Trivia: In the scene where Darth Vader and the Emperor are looking out onto the unfinished first Death Star, keep an eye out for a young Grand Moff Tarkin (played by Peter Cushing in "Star Wars").
Trivia: Penelope Cruz played the same character in 'Vanilla Sky' as she did in the Spanish version of the same film, 'Abre Los Ojos'.
Trivia: When Megamind as Bernard is in the library with Roxanne, the book he is reading ("Megamind Unmasked") has a description on the back of a self-help book entitled "The Happiest Me I Can Be". This is the same book that Metroman read (shown later in flashback) that caused him to decide to cease to be a hero. (00:43:30 - 01:08:30)
Trivia: The sound of Vigo growling is exactly the same growl that the terror dogs from the first Ghostbusters make.
Trivia: While on the helicarrier, Tony Stark is wearing a Black Sabbath T-shirt, the band who released the song "Iron Man".