Continuity mistake: Indians are on a hilltop and a jet vapour trail can be seen in the sky.
Factual error: Near the end of the film Butch is complaining about the living conditions they have to endure - jungles, swamps, snakes, night work - and Sundance sarcastically retorts "Bitch, bitch, bitch!" In 1908 the term meant just what it literally means: "Female dog." It did not adopt its current meaning of "complain" until much later. At the time the film is set - outside the context of "female dog" - it was considered to be a serious obscenity, and it would not have been used to describe something as ordinary as someone moaning about his living conditions.
Visible crew/equipment: In the last scene, when they are riding alongside a river, shortly after the harmonica player changes songs, there's a closeup of John Wayne and appearing from the right on the other side of the river, a pickup truck appears, and then they quickly cut to different characters. (01:56:55)
Visible crew/equipment: In several scenes there are rectangular arrays of studio lamps visible, reflected in the actors' space helmets.
Continuity mistake: At the end of the town collapse, a sincerely drunk Ben dives head-on into a tub where a lady (in her underclothes?) is just enjoying a bath. Propelled by Ben's momentum the tub with both passengers slides down the slippery planks and splashes into the river, while Ben rearranges himself into a comfortable sitting position between shots. If I wasn't completely mistaken also the lady changes her outfit, since the embroidery is missing when they float along. (02:23:35)