Continuity: When the T-Rex is going after Larry, Larry is behind a desk. The T-Rex bangs his head on the desk and then lifts the desk and throws the desk. A few scenes later the desk is back in place and there is no mess from the papers that were there before.

Continuity: When Larry notices the caveman's footprints leading to the open window and goes to investigate, the footprints are gone in the next shot.

Continuity: When the Neanderthals throw the foam on him, it noticeably changes shape and size throughout the next few shots.

Continuity: When Larry first meets the Easter Island head, he is talking to him from about 5 feet (or more) away. When it cuts back to him after the shot of the people chasing him, he is just inches away from it.

Visible crew/equipment: When Larry and Rebecca (in her red coat) are outside the museum and she is giving him information about the exhibits, there is a boom mike visible in the top part of the screen for a brief moment after they cross the street.

Revealing: In the park after the bad guys have been defeated and everyone starts returning to the museum, watch the T-Rex closely. In the overhead shot, he seemingly "floats" right through the branches of the trees which he normally would be moving.

Continuity: When Larry does the magic trick with the disappearing handkerchief, the amount of coloured handkerchief visible out of his thumb changes massively between shots.

Continuity: When Larry is explaining to Rebecca how the museum comes alive at night, her purse straps are alternately crossed and uncrossed on her shoulder.

Continuity: When Larry tells Rebecca that the museum comes to life, they are in front of the Neanderthal display. But when Rebecca leaves, they are in front of Teddy Roosevelt.

Continuity: When Larry lifts up the cowboy and the roman emperor and places them on the bench, they move closer together between shots.

Factual error: During one of the first scenes with Teddy Roosevelt he has a small "2" on his collar above his Cavalry crossed sabers. However, his "Rough Riders" were in the 1st Cavalry Regiment. Later in the movie he's wearing the correct "1" there.

Continuity: The epaulets on Larry's normal jacket disappear and reappear between shots.

Continuity: When Larry is talking to Erica about getting a job, they both drink glasses of water. In one shot, Erica is just about to drink from her glass, but in the next shot her arm is lowered down by her waist.

Continuity: In the end, when Larry meets up with Teddy Roosevelt, during that whole scene, the people in the background change positions, especially the guards in the blue cape.

Revealing: Towards the beginning of the film when Ben Stiller looks at the photo at him and his son, you can see that the photo is a fake. Ben and his son cast a shadow on the shadow behind them.

Continuity: In the scene when Larry is in the locker room getting ready for his shift he doesn't have a necktie on. As the shot changes when he turns to talk to the three guards and close his locker, he has a necktie on.

Continuity: When Rebecca leaves the Museum after sitting in front of the Sacajawea display after Larry asks her to leave as he's closing up, from the internal shot of when she's leaving the Museum she exits through the middle rotating door but on the outside shot she is seen leaving from the door on the right hand side of the Museum.

Continuity: When Cecil is giving Larry his equipment, he hands Larry the flashlight pointing down. In the very next shot, when Larry takes it, the flashlight is pointing up.