Evolution (2001) - 21 mistakes
Directed by Ivan Reitman, starring Dan Aykroyd, David Duchovny, Julianne Moore, Orlando Jones, Seann William Scott
Continuity: During one of the final scenes in a laboratory, some of the characters are talking about finding 500 gallons of selenium to help stop this evolution. When they ask, "Where are we going to find 500 gallons of Selenium at 2 o'clock in the morning?" there is sunlight coming through the trees outside the window.
Factual error: At the end of the film, when Duchovny and Jones are on the ladder truck shooting the head and shoulders, they are yelling "go go go". They slide down the ladder and the next shot is the fire truck speeding away. Movie elapsed time 5 seconds. It is a physical impossibility for a fully extended ladder to be lowered, retracted and bedded in a span of less than 4-7 minutes. Even if the hydraulic lines are cut, the ladder would remain upright and not collapse/retract.
Continuity: During many scenes of the movie, you can notice that Orlando Jones has no glass in his glasses. In fact, when they are in court, he puts his fingers through the frame to rubs his eyes. [Some people have said they're meant to be fake in order to make him look clever, but they've definitely got glass in some scenes.]
Revealing: This film was shot in Page Arizona with is pretty far north in the "high country". When they first drive up to the meteor side, there is Saguaro cactus that are only a couple of feet high with huge arms and a neon greenish color. These only grow on the desert floor in Arizona and they certainly are not neon colored. Additionally, the arms don't start appearing until they are much taller as they grow them to balance themselves since they have a very shallow root system.
Continuity: At the end of the film when David Duchovny and Orlando Jones go up the ladder to squirt the Head and Shoulders into 'the hole', Orlando Jones gets sucked up into the alien and when he is pulled out he is covered in slime from head to toe, (including his glasses), as is David Duchovny. However, in the next shot of them going down the ladder there is no slime on them at all. In the next shot when they are standing at the bottom, while the truck pulls away, neither of them have much slime on them, and Orlando Jones' glasses are completely clean. The amount of slime they are covered in changes throughout the next few shots.
Factual error: The aliens are supposedly nitrogen-based, making them vulnerable to selenium in the same way humans are vulnerable to arsenic because we're carbon-based. However, nitrogen atoms don't have the valence real estate to form the long molecular chains critical to the DNA structure shared by terrestrial and alien life (when Ira first analyzes the sample, he finds DNA with 10 base pairs).

