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Plot hole: Gardez and Michelle Carey both disappear with no explanation. While Gardez's disappearance benefits the plot, in order to introduce Jack, there's never any mention of why he's gone, be it personal/medical leave, reassignment, etc. Ditto Michelle: she was so determined to learn the truth about Tru, so her disappearance doesn't make sense.
Continuity: The first time Tru lives the day, she has breakfast with her brother first thing in the morning. Harrison goes to put sugar in his coffee and the lid falls off. The second time, they go out together much later (after Tru goes shopping with Lindsay), and Tru manages to grab the sugar lid just before it falls off. But people have been eating at the restaurant all day, so it's not at all likely that the lid would still have been loose. Plus, the same patrons are seen in the diner even though it should be a completely different crowd.
Continuity: The second time the day happens, the man stands up in the restaurant to profess his love to his girlfriend almost immediately after the waiter drops his tray. There is only about ten seconds between the two events, but the first time it all happened, there was a gap of nearly thirty seconds.
Plot hole: When Marco brings Andrew's corpse to the morgue the first time the evening happens, he says that Andrew was died "in his ex-wife's house." However, during the second instance of the day, it is eventually revealed that Sarah is actually still married to Andrew, and they are just separated. Sarah may have lied to Harrison about that, but the police would have gotten accurate information. In fact, when Sarah calls the police (the second time), she refers to Andrew as "her husband."
Plot hole: When Lindsay brings Tru breakfast in bed on the second version of the day, she is excited that Tru remembered her birthday, and she also mentions that nobody else did. But it's still very early in the morning. It's not at all reasonable to assume that anybody forgot her birthday just because they didn't call before 8am.
Plot hole: John is slightly surprised to find that Tru is acting as bartender for the party, but doesn't dwell on it any further once she explains that she was supplied gratis to make up for the earlier "problems" with the room. But John should actually be extremely alarmed to find her there, given that he poisoned the ice and expects the other guests to drop dead. He certainly wouldn't want any witnesses to that.
Plot hole: Meredith submits to the drug test at her office. In the next scene, which takes place later the same evening, Harrison tells Tru that Meredith's test came back positive and she was immediately fired. But it takes at least 24 hours to get a result from a urine test at all. If the test is positive, the sample must be subjected to a more thorough examination. It usually takes at least 72 hours to get the results in that case. There's no way Meredith's positive results could have come back in less than six hours, even if a lab had been open late in the evening.
Factual error: Tru arrives "more than three hours late" for the MCAT and then begins the test. Firstly, it would be pointless for her to even bother going at that time, since she'd have already missed the entire Verbal Reasoning section and possibly the Physical Sciences as well (depending on how much more than three hours she was late). Secondly, that's really a moot point since late arrivals to MCAT testing centers are not permitted to take the test.
Plot hole: Around the middle of the episode, Paige's father tells Tru that all Paige really remembers about her molestation is "the moon and stars," which doesn't make sense to anybody. Towards the end of the episode, Tru looks across the street and sees that Paige's neighbor has a large stained-glass window of the moon and stars, and immediately realizes that the neighbor is the one who molested Paige. Paige is already there, because her near-death experience opened up the memory for her. But why didn't anybody figure this out before? The moon and stars window has been in plain sight to Paige and her father for twenty years, and evidently Paige has been ranting about "the moon and stars" for some time now.
Revealing: It was raining in Vancouver the day the outdoor scenes were filmed for this episode, so the actors were filmed under large tarps. When Tru and Paige walk together in the park, rain can occasionally be seen in the foreground and background, but not over the characters themselves. The ground is also wet everywhere throughout the episode.