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Revealing: In the scene where Ronnie visits Brundlefly for the second time we see Brundlefly walking on the ceiling and wall, however his shirt doesn't remain consistent with gravity. This is especially obvious when he pulls his shirt up to show Ronnie his new tumor and then places it back down. The shirt appears to be being pulled up to towards the sky from Ronnie's perspective.
Character mistake: Brundle's steak experiment has one major flaw in its planning: he takes care to separate the two cuts of meat by placing them on different coloured plates so he knows which is the control cut and which is the teleported cut, however he then proceeds to cook them both at the same time in the same pan. Being a scientist you'd think he'd know this could quite easily ruin the results of the experiment as whatever changes occurred to the teleported steak could leak out with its juices and contaminate the control.
Continuity: When Seth cuts the steak in two pieces, one half is larger than the other. He then puts the larger half on the white and brown plate to teleport it. After cooking both pieces, he puts that larger half on the white plate. Seth then gives the smaller half on the white and brown plate to Veronica and tells her it's the piece that was teleported. Furthermore, when Seth cuts a very small piece from the larger half to give to Veronica, the next shot shows two remaining pieces on the white plate although only one piece should remain.
Visible crew/equipment: After Seth Brundle has gone through his final stage of metamorphosising into the Brundlefly, he throws Ronnie (Geena Davis) into one telepod and then starts to make his way across the lab towards the other one. Watch as he emerges from behind a wooden pillar, there's one shot where a crew member's arm is seen reaching inside the back of the Brundlefly puppet, to move the creature's head. You'll need to slow-mo to see it but it's there. (As per the rules of this site, visible crew members is an acceptable use of slow motion.)
Continuity: When Geena Davis gets kidnapped by the Brundlefly from the hospital, he gets into her hospital room by crashing through a plate glass window. When he leaves through said window we see that a portion of the left side has broken in half and is severely cracked. When John Getz's character attempts to stop them he smashes his hand through the same window section that's somehow become immaculately whole again.