Continuity mistake: When Rose gets into one of the hospital's elevators early in the episode, stock footage of an elevator descending is shown. When the Doctor, Rose and Cassandra (possessing her) go into an elevator shaft later on, it looks and is shaped completely differently. (00:34:05)
Audio problem: During the climax, when the Doctor has Wilf's old service revolver and is alternately pointing it at the Master and Rassilon, there's a cocking sound every time he turns. Not only is the handgun a revolver, so it only needs to be cocked once, the Doctor is only shown actually cocking the gun once, when he first points it at Rassilon. (00:44:10 - 00:45:55)
Continuity mistake: The episode repeats the closing scene of "Doomsday", when Donna unexpectedly appears in the TARDIS. However, several details are different. One, the console room is more brightly lit than in the previous episode. Two, the shirt belonging to Rose that Donna finds draped over a railing was not present. Three, the Doctor's coat (draped over a ceiling support) has shifted in place, despite not being touched. (00:01:15)
Continuity mistake: On the Valiant's bridge, when the Doctor is telling Martha and Jack about his plan to slip one of the perception filter keys around the Master's neck while holding his key, the position of the key changes in his hand, from being held by the wide end to being held by the narrow end. (00:37:15)
Deliberate mistake: The Globe Theatre is shown staging performances at night. In actuality, they performed during the day, when there was enough light, since this was pre-electricity. This was done both to make the climax of the episode scarier and because they shot on location in the real Globe, which still shows its plays during the day, and thus they didn't have a lot of time to shoot during the day there.
Continuity mistake: Among the various contents of the Black Archive are visible several objects that shouldn't be there, because it is impossible for U.N.I.T. to have acquired them. In particular: A detonation pack from "Planet of the Ood", set in the 42nd century, a few thousand years in the future. The Supreme Dalek's shell from "The Stolen Earth"/"Journey's End" - it was shot and killed by Jack Harkness, destroying the shell. The restraint chair from "The End of Time", last seen on the Vinvocci's ship. The makeshift sonic screwdriver built by the alternate Amy from "The Girl Who Waited", since that version of Amy was wiped from existence. A different explosive carried by Gregor Van Baalen in "Journey to the Centre of the TARDIS", which is both set in the future and involves a complicated situation in which the events ended up technically not happening.
Continuity mistake: The registration of Milo and Cheen's car is shown on the computer screen and stated aloud as "4-6-5-◊-6." However, there is a shot of Martha and Cheen looking out the window where the car's registration sticker is visible, showing a different registration: "9-9-6-γ-1." (00:13:15)
Continuity mistake: When the Sphere Chamber is first shown, there is a rolling staircase set up just below the Sphere. Rajesh Singh is shown climbing the staircase and nearly touching the Sphere. However, when the room is next seen, when Raj hears that the Doctor is coming and walks up to look at the Sphere again, the staircase has vanished. Later, when the Doctor and Jackie are shown into the room while Yvonne gives them the Torchwood tour, the staircase is still missing, and it's more obvious that it is no longer in the room. It does not reappear for the remainder of the two-parter.
Other mistake: Shortly after everyone arrives on the Vinvocci ship, when the Vinvocci and the Doctor run through the door on their way to the flight deck, the Doctor comes back to get Wilf, who didn't initially follow because he was mesmerized by the sight of the Earth from orbit. It's obvious that David Tennant went just behind the door and paused briefly before coming back into the room, which is not what the Doctor is supposed to be doing. (00:16:00)
Continuity mistake: During the scene on the hospital roof, when the Doctor is telling off the Atraxi while simultaneously choosing a tie out of several he stole from the locker room, the number of ties draped around his shoulders and the position of his hands changes between shots. (00:52:35)
Continuity mistake: In this episode's TARDIS scenes, the yellow padded seat is positioned so that it's on the side of the console closer to the door, so people sitting on it are facing away from the door. In all other episodes featuring this version of the console room, the seat is positioned on the side of the console opposite the door.
Factual error: The Olympic torch relay is presented as if there is only one runner carrying the torch through the entire city of London to the Olympic stadium to light the cauldron. Furthermore, when the torchbearer collapses, the TV announcer reacts as if it is now impossible to get the torch to the stadium, just before the Doctor steps in and picks it up. Any broadcast of any Olympic opening ceremonies would show that that's not how the torch relay has ever worked.