Continuity mistake: Kirk and the others are beamed down to the planet, and when the Enterprise crew are led into Mudd's throne room by two Alice androids (the Alice series are 500 identical androids) they are wearing the usual metallic, backless, kitten heel slip-ons, but in the next shot android Alice at the left is now wearing altogether different shoes - cream color with ankle-straps. (00:09:30)
Factual error: Trelane has been watching Earth as it was 900 years ago (14th century in the film), but his style is more 17th century, and he mentions Napoleon who lived in the 19th century.
This Side of Paradise - S1-E25
Continuity mistake: When Kirk and Spock successfully activate a subsonic transmitter, it cuts to a closeup of a spade's metal blade breaking the soil ground, but in the next wide shot Sulu and DeSalle are using two spades made entirely of wood. (00:44:45)
Visible crew/equipment: When McCoy gives the guard a shot and he and Scotty break into Engineering, you can see a crew member's hand holding a red handle and their bare arm (it's not Scotty), who apparently is helping to open the door. (00:23:00)
The City on the Edge of Forever - S1-E29
Continuity mistake: At the end, Kirk says "Let's get the hell out of here." Captain Kirk and 6 others were beamed up at the same time, but the Enterprise transporter only has 6 pads; presumably a red-uniformed crew member was lost in space.
Suggested correction: Short version is, the Enterprise has more than one transporter room. The Animated Series references at least four on the Enterprise from this era. There are also cargo transporters, which aren't normally calibrated for personnel, but can be, albeit at reduced capacity and increased energy usage. https://memory-alpha.fandom.com/wiki/Transporter_room.
Continuity mistake: In a wide shot of the Capellons and the Klingon walking down the hill, the Klingon trips and falls to the ground and picks himself up. Next shot, tight group with Klingon, he is suddenly brushing off his pants.
Suggested correction: After Kras falls, the next shot you see him still on the ground and starting to stand up, although Maab blocks the view of him. The shot cuts to the rocks and then when it cuts back, you see Kras walk up and stand next to Maab, then he starts to dust himself off.
Suggested correction: Why would he not be doing that in the next shot? There is no continuity issue.
Tomorrow is Yesterday - S1-E20
Character mistake: Kirk introduces Spock as "Lieutenant Commander Spock," but Spock is obviously wearing full commander's stripes on his uniform.
Visible crew/equipment: After Uhura asks McCoy if she'll become like Chekov, McCoy reassures her about finding an antidote. Then, when it cuts to Engineering, there's an actor's black tape mark and a chalk T-mark visible on the floor. (00:38:10)
Continuity mistake: When Kirk beams down to the planet, he selects the front transporter pad next to the steps, and closest to the door. When he materialises in the replica transporter room, he is on the same pad. When the scene cuts to a view of the empty transporter room, Kirk is no longer on the front pad. The scene cuts again, and Kirk steps off the front pad to walk to the control panel. (00:00:30 - 00:01:30)
And the Children Shall Lead - S3-E4
Audio problem: When Spock asks Kirk, "Why are they bothering Starfleet?", there is a loud buzzing noise twice as they are focused on Kirk's face.
The Conscience of the King - S1-E14
Continuity mistake: When Spock explains to McCoy his theory of Kodos' use of eugenics, the background behind Spock is a wall of red grating, but the shots of Spock speaking to McCoy alone (McCoy isn't in the shot) show a blue background wall with a control panel on it. (00:21:55)
The Conscience of the King - S1-E14
Continuity mistake: As Kirk asks the Enterprise's computer about the history of Anton the Actor and Kodos the Executioner, his hands change positions from being away from the computer's controls to near them, then clasped as the shots quick cut.
Who Mourns for Adonais? - S2-E2
Continuity mistake: In the original version, the giant hand is holding the Enterprise for most of the episode, but when the Enterprise fires phasers at Apollo's temple, the hand is gone.
Continuity mistake: When Scotty thwarts Garth, disguised as Kirk, attempts to beam to the Enterprise using the Queen-to-Queen level three code, Kirk throws a tantrum and accidentally loosens one of his controls during his rant right before he turns back to Garth.
Suggested correction: How is that a mistake?
Continuity mistake: When the Enterprise tries to escape Gothos, and the planet blocks the ship's path each time, Gothos is very transparent, where the stars could be seen through the planet.
Continuity mistake: After Spock nerve pinches Chekov when he goes mad and he is taken to Sickbay, the Tholian ship appears and hails the Enterprise. When Spock says "on main viewer," the shot of Loskene is on the screen, but the back of Chekov's head can be seen when it should be in Sickbay.
A Taste of Armageddon - S1-E24
Continuity mistake: At the end of the teaser, Kirk orders Yellow Alert, but the Red Alert alarm sounds.
The Alternative Factor - S1-E28
Other mistake: When Spock calls Kirk to the bridge after he discovers the Lazarus energy source, he and Lazarus head to the bridge. There is a shot of McCoy standing in the corridor, and one of the red alert panels is burned out while the other is still flashing.
Is There in Truth No Beauty? - S3-E5
Visible crew/equipment: Spock and Dr. Jones carry Koldos into his quarters, and when Spock walks over to the wall communicator to contact the bridge, the camera casts a shadow on the wall as it moves closer to Spock. (00:05:20)
Is There in Truth No Beauty? - S3-E5
Visible crew/equipment: At the dinner, when Miranda stands up to leave, there's a visible white tape mark on the floor near her feet. Then, when McCoy makes his exit Kirk reaches for the rose, and there's a better view of the white tape mark under the table. (00:11:50 - 00:13:45)