Revealing mistake: In many episodes, when the outside of Mario and Luigi's business is shown, the edge of the backdrop can be seen.
Other mistake: In many of the live-action segments, the Alarmtron logo on the alarm in the Mario Bros' residence has been partially covered to read "Acarmtron" instead. In addition, in several episodes, the phone number for Alarmtron is also partially covered.
Revealing mistake: When the Mario and Luigi in real life appear from the left side of the screen, the green or blue screen is not formatted properly, causing them to appear out of nowhere. (00:00:43)
Continuity mistake: This mistake is in the intro with Mario and Luigi in real life. When Mario breaks the ceiling while Bowser is holding a yellow bar, Mario's gloves disappear from his hands when he jumps to break the ceiling. When he lands, his gloves reappear back on his hands. (00:00:26)
Continuity mistake: When Mario moves a curtain in the animated intro, there is no cavity in the shower, but in the next shot, one appears. (00:03:35)