Continuity mistake: When Dr. Proctor says that he has been unable to refuse requests from young girls, the yellow clips on Misty's suspenders turn red like the suspenders. (00:03:22)
Other mistake: When Pikachu's pain increases, his nose is missing. (00:01:51)
Continuity mistake: When Ash, Brock, and Misty rush to find a Pokémon Center for Pikachu because he has fainted, they leave their backpacks behind. When they arrive at the hospital, they suddenly have their backpacks with them. (00:02:26)
Continuity mistake: When Ash realises that something happened with Pikachu, his bag is below the second-highest plank on the bench, but in the next shot, it is aligned with it. (00:01:27)
Answer: Yes it ran away because Layla hurt its feelings.