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After Leon beats Bitores Mendez and takes his fake eye, you will notice if you look closely at his corpse that his fake eye is still in his head.
After leaving the main menu of RE4 for about 20 seconds, the trailer for the game comes up. During the course of the trailer, there is a short scene of Ashley putting her head in her arms. This is a reverse of a cut scene in the game where you rescue her after fighting the Iron Maiden, in which she looks up to see Leon.