Factual error: When the real German soldiers are playing 20 questions in the bar, one of the cards shows the name of Genghis Khan. This is an English-language word; as Germans, they would have written Dschingis Khan instead. As the whole scene deals with exposing the British/American spies and subtle differences in their language or behaviour, this is especially unfavorable here.
Continuity mistake: In the scene where Col. Landa hands himself over to Lt. Raine, Utivich cuffs Col. Landa. Then when Lt. Raine is carving the swastika into Col. Landa's forehead, you can see him clutching at grass with his right hand, which isn't cuffed.
Continuity mistake: In the final scene between Brad Pitt and the Nazi officer before he carves the swastika into the German's forehead, Pitts' tie changes three times. First scene it is on both sides of his neck. Second scene it has disappeared from the right side, and the third scene, it is back completely around his neck again.
Continuity mistake: In the scene in which Colonel Landa and Shosanna are talking in the cafe, Landa orders an espresso for himself, along with a glass of milk for Shosanna and two strudels. When the order arrives on a tray, the cup of espresso is seen briefly in one shot. The film then cuts to a close-up of the tray, and the espresso has disappeared, despite no-one having put it down. In the next shot of the table, the espresso has appeared beside Landa.
Continuity mistake: In the Operation Kino briefing scene, in the shot where General Fenech and Lt. Hicox drink to "down with Hitler", Fenech is holding his staff in his left hand along with his blue file folder. In the next shot, as Fenech asks Hicox if he is familiar with German cinema under the Third Reich, the staff is suddenly no longer in his hand, but being held pinned under his left arm. In the following shot, Fenech is instantaneously once again holding both the folder and staff in his left hand.
Continuity mistake: In the tavern scene, when the SS officer tells Lt. Hickox that he is no more German than the scotch they are drinking, Hickox is sitting back against the back of his chair. In the following shot, which shows Eric the bartender slowly reaching for his shotgun, Hickox can be seen in the background leaning forward in his chair. In the next shot, Hickox is suddenly once again sitting back in his chair as he was before. It is implausible that Hickox would have or even could have changed seating positions so quickly or abruptly.
Continuity mistake: When Hans Landa first approaches Bridget von Hammersmark in the theater, he is shown to be walking right up to her, as evidenced by the shot of his right foot being shown to be mere inches away from her left leg. When the shot changes to a medium shot of von Hammersmark saying "Colonel Landa, it's been years," Landa is suddenly out of shot, whereas the proximity of his feet to von Hammersmark's suggests he should have been visible in the medium shot.