Revealing mistake: At the end when the dock 'kicks' the king spider off him into the fire, we hear it scream like a stuck pig. Then additional screams from the 'burning' spiders inside the pulsating nest, and when the doc torches both the king spider afterwards.
Suggested correction: Not sure how this is a mistake. Sure real world spiders don't making squealing noises but real world spiders also don't jump 30 feet or have venom potent enough to kill a human within seconds of being bitten.
It's a mistake because spiders cannot vocalize at all. The film creates a new spider species with exaggerated aspects of real life spiders: extremely potent venom, highly aggressive behavior, jumping ability, etc. What the film can't do is give the spiders traits they couldn't possibly have in real life.
Factual error: Towards the end Ross pours wine on the egg sac to light it. Wine doesn't contain enough alcohol to ignite.
Suggested correction: He uses cognac and not wine, which is why it burns.
Corrected entry: The spider specialist should have known that when he vibrated the web he would be attacked by the giant spider, but still he did it. (01:04:02)
Correction: The spider specialist wanted to get the giant spider out in the open so he could take a look at it. He expected it to come out and walk along the web so he could study it, not leap out and kill him like it did. The spider attacking him was an accident.
Correction: He was about to see something spectacular, I put it down to him being foolish in the moment and paying the price.
Continuity mistake: When they perform the autopsy on the three victims, Metcalf is all grey and stiff, whereas the two exhumed victims look alive and fresh.
Suggested correction: Not that what you said is wrong, but it actually makes sense. Metcalf was bitten by the original, non-bred spider. The other victims were bitten by spiders that were part house spider, part deadly spider. Therefore, the effects of the bites were different. I'm quite glad that I only had to see one gray, shriveled-up body.
Actually all three were bitten by hybrids.