Continuity mistake: After the gas explosion in the school, Angela reveals that Alice is infected. The woman cop asks "When were you gonna tell us that?" and the next shot shows Alice with burns/blood on her upper left arm. In the next shot as Alice goes to sit down, the severity and position of the burns/blood changes.
Continuity mistake: Watch the left side of the mouth piece in Alice's mouth when she is in the tank at the end. In the shot up close to her left there is a plastic support going up, in the next shot of her right the support is pointing down, and after the scientist says "can you hear me?" and the shot is close to her, the support isn't even there.
Continuity mistake: Toward the end, Major Cain storms aboard the helicopter, shouting "Why haven't we taken off, yet?" The shot switches to LJ (Mike Epps)in one of the pilot's seats. As he says, "Because I normally drive Cadillacs" he punches out at Cain with his left hand. In the reaction shot, however, as Cain flies backward, the punching hand is LJ's right hand.
Continuity mistake: When Alice and the group are going to raid the Umbrella guards and steal the helicopter, one of the men looks through binoculars with night vision. He looks at the helicopter, then looks to the right of the helicopter, yet in the next scene a second later, his head is in the same position as it was when he looked at the helicopter, before he looked to the right.
Continuity mistake: When Jill Valentine and Angie are running from the dogs after turning on the gas, Jill kicks the left side of the double doors open. However, when she turns around to see if the match ignited the gas, she has the right door open. When Jill Valentine realizes Alice light a cigarette she starts running, again out of the right side of the door. (Looking at the doors from the kitchen to the cafeteria).
Continuity mistake: In the DVD, at the climax, just prior to when Alice is forced to fight Nemesis, she has a conversation with Thomas Kretschmann (the dude with the leather coat). During the conversation, one shot shows Alice with blood trickling down her neck and some bruises on her face after she previously had no injuries. Moments later, her face is back to normal. Later, during the fight with Nemesis, you see that that is where she sustains her injuries.
Continuity mistake: As Alice is attacking the guards around the helicopter with the batons, she switches from holding them low to holding them high between shots right after she knocks down the last guard. There isn't enough time or any reason for her to do so in the second offscreen as the others arrive.
Continuity mistake: In the church, Peyton asks Terri about her video camera. She has it raised to shoulder height. Cut to her face and the camera's gone. The next shot is through the camera and shows the same view of Peyton we could see before over her shoulder, so she didn't move it.
Continuity mistake: In the church scene, Alice blows up her motorcycle with two shots of her Colt .45's. During the shot of her back, she already starts with holstering her guns, her arms are already halfway there when the shot cuts. Then, there is a quick shot of the explosion, and in the next shot her arms are straight again, in the position where they were when she took a shot at her bike. There is no reason or time enough to do that.