Other mistake: In the slaughterhouse scene, after Harrigan has injured the Predator with his shotgun and Keyes reappears, the Predator throws its disc, and severs Keyes in half at the waist. We see his legs flop to the ground, and blood pour from above, but his upper half (torso, backpack and weaponry) mysteriously remain hovering out of sight.
Revealing mistake: When the subway train slams on the brakes, the camera cuts to a shot showing the sparks coming off the wheels as the train is slowing down. If you look at the wheels carefully, you can see that the train is already stationery, and the camera is panning down the train to give the impression its slowing down.
Deliberate mistake: When King Willie is confronted by the Predator in the alley the Predator approaches him cloaked stepping through the puddle of water. You can see his footsteps causing the water to splash up. However, you can see the water splash up and go through his foot. The Predator is only cloaked, not intangible so the water should have splashed upon his foot, not go through. Water against his foot would be visible too, even though he is cloaked.
Continuity mistake: At the penthouse, the Predator lifts one of the Jamaicans on his wrist blades. Another Jamaican turns to see his friend dangling in the air with a claw sticking through his torso. In the next shot from the Predator's POV, the Jamaican is facing as before and still shooting at the wall like he didn't notice. After that, it shows the same man turning in an arc that should show the Predator, but it and the impaled man have disappeared entirely.
Continuity mistake: At the end of the fight in the slaughter house, Lt. Harrigan scrambles up a ladder and goes onto the roof with the predator in hot pursuit. A few moments later, Harrigan slams into the predator and they both fall off the roof. Suddenly, they are about 30 stories up dangling from a ledge. The slaughter house wasn't 30 stories tall...it wasn't even three stories!
Revealing mistake: At the very start of the film, when the car blows up, if you look to the right you can see a piece of debris (it looks like a car door) flying through the air. Look on either side of it and you can see the black cable it's gliding on.
Revealing mistake: When the predator fires his plasma caster in the slaughterhouse scene, you can see that the simple laser blast has been replaced by a Chinese sparkling firework on a string. In the original "Predator" you could see this same effect on a string but it was not nearly as spectacular and easier to miss.
Continuity mistake: In the beginning, after the gang retreats into the building, you see what appears to be the leader sniffing from a golden container. After a quick cut to the gang getting ready, you see his moustache covered with white powder. After another cut, a closeup shows his moustache is clean, way too quick to have cleaned up.
Continuity mistake: At the start of the film, Glover's character blows away the Colombians with a shotgun outside their drug den. Immediately afterwards we're shown the Predators POV (in thermal vision) - looking directly down at their bodies, which show up a greenish color. This would indicate their body temperatures dropped to a much lower level impossibly fast.