Factual error: When Janet Leigh is shown lying dead on the floor of the shower, there is a close-up of her open eye. The pupil is contracted to a pinpoint (obviously due to the bright lighting) where it should have been dilated. After the film was released, Hitchcock heard from several ophthalmologists who pointed this out and suggested he use belladonna eyedrops in the eyes of "dead" people in future films, as the chemical prevents the pupils from contracting.
Continuity mistake: When Marion arrives at the Bates motel, she sounds the car horn for the first time, the windscreen wipers are switched on. She gets out of the car, looks towards the old house, then goes back to the car, as she sounds the horn for the second time the windscreen wipers are now switched off.
Continuity mistake: In the shot in which Norman goes into the bathroom and puts the pail behind the door, the shower curtain rings are spread out. In the very next shot in which he emerges from behind the door, the rings are in tight groups. As he leaves the bathroom, the rings are spread out again.
Other mistake: In the shower scene, Marion Crane's palm looks water wrinkled as if she's been in the shower for quite a long time even though she was only in for a few moments before Norman stabs her. Also, when Norman drags her out of the bathroom, Marion's legs are stiff and together and she has her toes pointed.
Continuity mistake: After seeing Marion's dead body in the bathroom, Norman accidentally knocks down a framed picture of a bird (with the picture facing up and it is a few inches away from the wall). But when Norman is spreading the shower curtain on the floor, the picture has changed position (with the picture now facing down and it is against the wall).