Factual error: At the very beginning of the episode Leonard says "So you see what you're eating is not technically yogurt because it doesn't have enough live active acidophilus cultures." He is implying this is a scientific fact (he's not just giving his opinion on yogurt.) But to meet the U.S. standards for yogurt, only the starter cultures Lactobacillus bulgaricus and Streptococcus thermophilus are required, not Lactobacillus acidophilus. The presence or absence of L. acidophilus does NOT determine a food's identity as yogurt (which Leonard's line is implying).
Factual error: Leslie Winkle takes shelter with Howard in the middle of a paintball game. Her gun has no hopper for paintballs and thus no ammo, so it's useless, despite her apparently using it outside seconds earlier. This isn't a model with an internal magazine - the port to attach the missing hopper is plainly visible.
Character mistake: When Sheldon arrives at the apartment with his Nintendo 64 in the box, he tells Leonard that they can play Super Mario 64. He then proceeds to say, upon finding a memory card, that they can pick up where he left off. Super Mario 64 does not make use of the memory card, all of the data is saved on the cartridge.
Revealing mistake: Near the end of the show, the big robot is chasing the other robot down the hallway. The camera starts to zoom in as the robots come by it. The big robot stops just before the camera zoom is past it, yet they keep running down the hallway like the robot is still going.
Continuity mistake: In the opening scene, just after Howard says, 'Creepy good or creepy bad?', there is a shot where Howard is holding his food, even though Leonard hasn't given it to him yet and it isn't there in the shot before or after. After Leonard gives the food to Howard, the exact same shot is used after he says, 'I'm not necessarily talking about the food', so the shot was taken from there and it is shown twice.
Continuity mistake: In the laundry room when Penny is talking to Sheldon, Sheldon takes some time arranging a blue shirt on his plastic laundry folder. Then it suddenly turns into a pair of socks, which he folds and then picks up the blue shirt again. There was not enough time for him to switch out the shirt for the socks.