For those who don't (or can't) read American Sign Language, at the end of the episode, Grissom says to Dr. Gilbet is that his mother lost her hearing when she was eight years old. He once asked her what is it like to be deaf, and she told Grissom (who loved to swim) that it was like being underwater. She also taught him that being deaf does not make one inferior to others. Dr. Gilbert then replies that she teaches her students the same lesson.
Anthony Zuiker chose to set the series in Las Vegas because that city's crime lab is the second most active in the United States, behind the FBI lab in Quantico, Virginia.
In most scenes where there is a phone call list, you might spot names like Michael Novotony and David Lindsay. They are the art directors of the show, and the faces that the AFIS scrolls through are people who work for CSI or are extras in the show.
Season 9, episode 20, titled "A Space Oddity"- In the scene where Simms translates the alien language that Hodges spoke to her earlier in the show with the Vellikon translator on the Astro Quest website, the alien alphabet that is shown on the computer screen is the same alphabet used in the Star Wars Universe, not Star Trek, which the show is modeled after.
When you see the CSIs use a website, none of them are actual websites. If you type in the website you will get redirected to CBS.com, the network of CSI.
Under the glass top of Grissom's desk are pictures of Jerry Bruckheimer, the shows executive producer.