Continuity mistake: During the opening credits, Frasier is playing the piano and Martin is going through papers on the table. When Marty says "I don't know, Eddie, must you?" there is something pink lying on the table. The shot goes back to Eddie and then back to the table, but the pink item has disappeared.
Continuity mistake: Towards the end of the show, Frasier is explaining to Niles how he was stood up that evening; at the same time he is getting himself a glass of sherry. Niles says, "there was no emergency", we see Frasier repeat the actions to get a glass of sherry all over again.
Revealing mistake: In the scene where Frasier and Lilith meet in the living room, where Frederick has created a romantic atmosphere, Frasier has a long speech about how Lilith is not going to get him back, ending with "I need to be finessed!" and Bebe Neuwirth goes out of character and begins to giggle, before she quickly covers her mouth and pulls herself together. Though Lilith might have found Frasier's comment amusing, it would definitely not be in character for her to giggle like that.
Continuity mistake: Frasier is just saying goodbye to Niles and we can see the brochure for Daphne's reception to the left of his sherry glass. When he stands up and shouts "Daphne!" the brochure has moved about 6 inches to the right, even though no one touched it.