Continuity mistake: In the scene where Gru first meets Lucy Wilde, Gru takes out his freeze ray and fires it at Lucy. Lucy stops the freeze with a flame gun thing and then she walks over to him and tazes him. Gru then drops the freeze ray, but it disappears when she goes to put Gru in the boot of her car.
Continuity mistake: After watching Eduardo activate the dance floor key to the lift, Gru then does the same thing. During the first attempt, Gru spills guacamole on the floor, his nacho hat gets sliced in half and then he throws the nacho hat on the floor. Gru then fails to unlock the lift so he tries again. Only now, the nacho hat and the guacamole have vanished.
Continuity mistake: The tattoo of the Mexican Flag on El Macho's chest vanishes after the bakery scene.
Continuity mistake: One of the minions is shown dressed as a maid hoovering the floor. The hoover continues to move along the floor after the minion answers the door. The hoover is shown moving past the door. But when the next minion is shown, the hoover is now shown only just going past the door.