Visible crew/equipment: When Pee Wee is chaining up his beloved bike you can see the long chain coming up through the bottom of the bike box. Full screen version only.
Continuity mistake: When P.W. is escaping certain trouble somehow his handle bar comes off (with ribbons) a new one is replaced (without ribbons) - cool bike huh? But in the next scene the new handle has ribbons...
Revealing mistake: When the pancakes are sticking to the ceiling, you can see that when one hits the ceiling, it jumps. The pancakes were actually being dropped on the floor with an upside down shot.
Visible crew/equipment: Near the beginning of the film, when Pee-Wee unveils his beloved bicycle for the first time by disabling his elaborate garage security system, if you look carefully, a crew member's foot can be seen moving away as the garage door is opened.
Visible crew/equipment: Look closely when Pee Wee and John the hobo are sitting on the side of a traincar singing and you can see safety straps keeping them on the train.
Revealing mistake: When Pee Wee is driving while Mickey is sleeping, he is passing all these signs that warn the road is going to become curvy. If you look at the bottom of the signs, you can see they are being moved on a track while the camera stays stationary. Full screen only.
Revealing mistake: After Pee-Wee tears through a Godzilla production in the Warner Bros. studios with his bike, he exits the stage, ending up on an alley where he turns left to evade the props and security guards on his tail - one of which has a motorbike. On the pavement beneath him is a curved skid mark from a previous take, not unlike the one left behind by the motorbike seconds later.
Continuity mistake: When Pee Wee gathers everyone together in his basement to discuss his stolen bike, there's a shot of his dog Speck sitting in front of a person wearing horrible plastic pink or orange boots. It's Pee Wee's friend, Dotty. In the next shot, Dotty comes down the stairs...
Continuity mistake: In the pet store fire scene, you see an aquarium full of fish. At one point Pee Wee stops to rescue them by scooping them into a plastic bag filled with water. While he's doing this, the aquarium is nearly empty. In the last scene of the sequence (as he's running out of the store with the snakes), the aquarium is again chock-full of fish.
Revealing mistake: In the beginning of the movie, when Pee-Wee's breakfast contraption is running, if you look closely at the "orange" that is being squeezed, it is really an orange coloured sponge ball.
Visible crew/equipment: When Pee Wee's getting his beloved superbike out of his secret hiding place you can see a hand holding it up for him.
Continuity mistake: When Pee-Wee is walking away from his house with his bike, you can see two lawn gnomes behind him, next to his house. When he sees Francis, the lawn gnomes have mysteriously disappeared.
Continuity mistake: Early in the Warner Bros. studio chase sequence we see a team of security guards in pursuit of Pee-wee. Somehow the guard in front switches from using a golf cart to a bicycle, all within a few brief cuts. This is the same guard who pulled the right handle from Pee-wee's bike moments ago.
Continuity mistake: In the scene where Andy chases Pee-wee down with a dinosaur bone, we can see Simone in the background flailing her arms and screaming Andy's name. At first she's walking towards a nearby staircase on the dinosaur's left side, but between cuts she quickly repositions and is now standing in front of the dinosaur's large foot instead.