Thor: Loki is beyond reason, but he's of Asgard and he's my brother.
Black Widow: He killed 80 people in 2 days.
Thor: He's adopted.
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Continuity: When the Helicarrier is about to take off, Banner and Rogers are still at the edge of the landing strip, identified by the yellow SHIELD logo and the jet that Banner is checking out. But the shot hangs on Romanoff for a second, and when it cuts, Banner and Rogers are instantly at the very edge of the ship, a good 50 feet away from where they were a second ago.
Deliberate "mistake": The Mark 7 takes 9 seconds to assemble - give or take. Judging by the view of the Chrysler Building, Stark Tower is about 300 meters high. Even if you wouldn't count the time it takes for the suit to catch up with Tony, 9 seconds is too long, he would hit the ground before the suit would fully assembled.
Factual error: In the scene after the chitauri army enters the atmosphere, they start shooting people in the ground and in the next shot if you see underneath the bridge there is a restaurant with people running around to avoid getting hurt. If you see there are two glasses of wine and some food plates sitting in a table firmly which does not even move despite the blast wave that was created from all the shooting in the ground near it.