Continuity mistake: When Flynn is being rescued by the "ruffians", after the second guard is taken, the head guard turns around and Flynn is still handcuffed. Then the head guard is knocked out, and immediately following the other guards break through the rear door and Flynn takes off running. His handcuffs are mysteriously gone. No one removes them.
Suggested correction: The Viking with the horned helmet does. Immediately after the guards break the door, three men are around Flynn (one of them hanging from the ceiling.) One of them is doing something with Flynn's hands.
Other mistake: Rapunzel's hair is somehow attached to the top of a tree and she is suspended off the ground, swinging in circles around the tree. However, her position relative to the ground does not change. As she repeatedly swings around the tree, she should be getting further above the ground. Also, her hair inexplicably never gets caught or snagged in the branches of the tree.
Audio problem: There is a clear lip-sync problem in the boat scene, after Eugene takes out the lanterns.
Continuity mistake: When Mother Gothel falls from the tower, her hands are yellow and young looking but in the previous scene her hands turned old and gray. (01:25:05)
Factual error: Sailing ships in harbor always have their sails furled and stowed, not set. All the ships in the city's harbor have their sails set. Would never happen.
Other mistake: In the boat scene, when Eugene takes out the lanterns, Rapunzel is moving way too fast compared to before and after.
Continuity mistake: In the reprise of Mother Knows best; Mother Gothel gives Rapunzel Flynn's satchel containing the crown in order to try and prove his disloyalty. Rapunzel then gives this to him on the boat later. However, no-one carries the satchel from the campsite to the boat! Rapunzel and Flynn are not carrying it, and Maximus does not have any saddle bags. How does it get from A to B?