Continuity mistake: In the scene where Buffalo Bill is about to take aim, his left hand is on the metal ring on the rifle. In the next close up of Wild Bill Hickok we can now see Buffalo Bill's hand has moved to another position further down from the ring and in the third shot, his hand is now covering the ring.
Continuity mistake: Before the ambush with the Indians, we see Buffalo Bill on a horse - only it's not him, but a stand-in. In the next shot we see Buffalo Bill closer up, and comparing the profile and facial structure of both shots, it's a different person on the horse to the actor that portrays Buffalo Bill. (Stand-ins were often used in older films when the actor was not available for filming for whatever reason.)
Continuity mistake: In this scene we see Buffalo Bill and Wild Bill Hickok in a shootout with the Indians. Hickok has a belt with bullets over his shoulder. The bullets start with nine from the top of the shoulder down, then a space of three then six more bullets. As the gun battle goes on he uses more, then we see a closer shot and now there are fifteen bullets from the top of the shoulder going all the way down the belt.
Continuity mistake: In the scene where Louisa Cody moves from the railing of the boat and stands at a doorway and looks into the room, to see her husband Buffalo Bill Cody is sitting at a table with others. In the very next shot she is back at the railing of the boat and looking back into the room.
Continuity mistake: In this scene we see several Government Officials talking in a room. One of them, while talking, is playing a game. The game is to balance the lid of an ink bottle on the end of a ruler and over the ink bottle. When the ruler is flicked rapidly gravity would do the rest and the lid would fall into the ink bottle. Things don't work to plan and the lid falls on its side. In the next wide shot, the lid is right side up and a little distant from where it fell earlier.
Continuity mistake: We see that Calamity Jane is behind the counter of a bar in a saloon. A man comes over and puts his hat on the counter at the base of a machine that mixes drinks and he orders an egg and sherry mixed. In the next shot there is no hat to be seen. As the machine begins mixing, the hat is back, and a short time later it disappears. Again as the camera angle pans back, we see the hat has moved from the original position it was in earlier on, to being further away from the base of the mixing machine.