The comic that Hannibal shows Blade, "Tomb of Dracula," is the comic in which Blade first appeared.

Ryan Reynolds gained 11kg of muscle for his role as Hannibal King.

In the Dracula shop when Dracula enters you can see the girl from the shop wears a Motorhead T-shirt. Motorhead sings the entrance song (The Game) of HHH.

In the scene where Blade's base is blown up, there is a shot of Blade being thrown into the air. This is not CGI or even wirework. When the director asked the pyrotechnicians to rig the warehouse, they decided to make the explosion bigger - without telling anybody. The resulting explosion threw the stunt double three metres into the air and nine metres backwards.

The whole vampire final solution plot line, with the humans in the big vacuum bags being harvested for their blood is actually an unused sub plot from Blade 1. In Blade 1, the vampire Frost was originally to merge with a Blood Tide and wipe out all humans on the planet. He had planned on the storage idea to maintain a food source for the vampire race once this was completed. This plotline was shown to test audiences who hated the ending (with Frost merging into a giant tornado of blood once the ceremony was completed). According to the directors commentary on the deleted scene on the DVD, the creators had to come up with a new ending and they chose the big sword match. They then dropped the humans as blood cows thing as it was no longer needed for the story. It was then resurrected, almost picture perfectly (based on the story boards for Blade 1 - again on the DVD) for this movie.

Jessica Biel inadvertently destroyed a $300,000 camera when she shot it with an arrow during a scene (she was directed to "aim for the camera"). Director David S. Goyer intends to include it on the DVD release.