Continuity mistake: When Blade has been rescued by Abigail and Hannibal and the 3 of them are approaching the hideout, notice in Abigail's back quiver there is one arrow left, yet in the next shot when they're walking up the stairs she has two.
Continuity mistake: In the scene where Hannibal King is chained to the floor in a cage, some shots show that the cage has bars across the top. When Blade drops through the window, he drops down through the top of the cage which no longer has bars on top.
Continuity mistake: After Blade shot the human, he walks up to where the human is lying on the ground. The male body lies to the left of the yellow line on the road. When Danica is looking at what she has recorded, on her computer's monitor, the body shown on the screen is now lying on the yellow line.
Continuity mistake: Towards the beginning of the interrogation between Dr. Vance and captured Blade, Vance's jacket is constantly buttoning and unbuttoning itself.
Audio problem: When the chief of police arrives at the blood farm he is surprised by Blade and Abigail. He quickly reaches inside his jacket to draw his pistol but Abigail draws her revolver much faster. As she draws there is the unmistakable metallic sound of a revolver being cocked, but when she extends the revolver out in front of her to cover the chief the hammer is down, not cocked. The revolver could be seen in the holster with the hammer down, so she could not have been manually decocking it to make that sound.
Continuity mistake: When Blade jumps through the police station window and lands on the pavement outside, we see a lot of broken glass from the window falling with him and landing all around him, yet when he stands up and says, "Forgot my sword," there is no glass to be seen anywhere.
Continuity mistake: Shortly after the Interrogation scene (when Danica, Asher, and Jarko walk in to meet Blade) Asher Talos begins to unlock Blade's hand restraints. Not only is his head up, then right next to Blade's face in the very next shot, but he also has a cigarette in his mouth when he begins. In the next shot the cigarette is gone, and does not reappear.
Revealing mistake: When Dracula is on the autopsy table, disguised as Blade, he can be seen taking a breath (look near the neck).
Revealing mistake: In the scene where Blade kicks Triple H and he goes through the wall, you can see the hole in the wall before he flies through it.
Continuity mistake: When Blade speaks with Dracula at the top of the building the sun changes in every shot.
Continuity mistake: When Danica and the others are inside the pyramid, Danica is holding her blue lantern at chest height but when the camera cuts to an overhead shot of the sinking sand, the lantern is being held down by the legs, at the left of the screen. (The blue light reflection is on the knee.)
Continuity mistake: In the beginning after the car chase, the helicopter spotlight that follows Blade when he runs away from the man he just killed is much different than in the video on the computer that the female vampire is watching.
Deliberate mistake: When Blade Abigail and Hannibal go to visit Dr Vance, Drake grabs Hannibal they move to a window, if you look behind them, the window is closed, then when Drake stabs Hannibal the window is open, then after drake goes out the window, you hear the window smash, even though he went out an open window.