The line "You are one ugly m*****" that Lex delivers to the Alien when she kills it before the exit through the shaft, is the same that Major "Dutch" Schaeffer told to the Predator in the original "Predator" (1987) film.

The way the bespectacled guy shines his torch on the eggs that have just popped up in the Sacrificial Chamber (illuminating the facehugger inside) echoes of the scene in the original Alien where Kane does the same thing to the eggs found in the hold of the ship.

The altars in the Chamber of Sacrifices of the pyramid are exactly arranged as the original hibernation pods in the Alien movie (1979).

With this film, Lance Henriksen has now been killed or otherwise assaulted by the title creature of three major sci-fi franchises; a Predator, an Alien and a Terminator. The only other current member of this fraternity of the unfortunate is Bill Paxton.

One of the taglines for this film is "Whoever wins...we lose." This is amusing as all the aliens and the predators in the temple are killed, leaving only one human survivor. I guess we won after all.

When Weyland is in his office near the beginning of the movie, he's playing with his pen. He starts tapping it on his desk between his fingers, alternating which two fingers he taps between. This is what Bishop does in Aliens (albeit with a knife) with his hand on top of Hudson's; maybe it was programmed into Bishop's intelligence.

Just before Miller enters the whaling station on his own, he cracks open a flare. The camera then does a shot behind the door before Miller comes in, but the light cast through the cracks in the door are a carbon copy of "Aliens", when the salvage ship scans Ripley's escape pod with the same effect.

There is a dipping bird in the background of the receiving station in Nebraska just like the one in the first Alien movie. Also, the morse code in the background says, "whoever wins. we lose."

Paul W. S. Anderson wrote the parts of Charles Bishop Weyland and Maxwell Stafford specifically for Lance Henriksen and Colin Salmon, respectively.

Colin Salmon's character starts to be cut into sections by the predator's net. Likely a nod to Resident Evil where his character meets a similar fate (lasers instead of a net), Both movies were directed by Wes Anderson.

Sebastian tells Lex how to say "scared shitless" in Italian: "Non vedo l'ora di uscire da questo piramide con te, perche mi sto cagando adosso." This literally means: "I can't see the hour to get out of this pyramid with you, because I'm shitting myself."