Continuity mistake: When Bond and Honey are rescued in the small boat, Bond rises and places his hand on the side of the boat. From the opposite angle, his arm is resting on the side.
Plot hole: It's assumed that Dr. No had Professor Dent slip the spider into Bond's hotel room that night but if it were that easy to do that then why not just hide an assassin in the room to begin with. Someone had to be in the room to tamper with Bond's briefcase, the closet, and possibly the bottle of liquor so just hide an assassin instead of waiting for the spider to kill Bond. (00:40:00)
Character mistake: When 007 is interrogating Professor Dent at Miss Taro's house, Dent attempts to shoot him when he asks "who are you working for?" But a minute ago, his gun ran out of ammo when he was shooting at the fake 007 in the bed. So there is no point of picking it up and trying to shoot him. Also, when this happens, 007 says "that's a Smith and Wesson. And you've had your six." This is incorrect, as Dent carried a Colt M1911. However, he did fire six shots before running empty, which is one bullet less than the magazine capacity of an M1911. (00:56:30)
Continuity mistake: At one point Honey is not wearing a bikini top under her wet shirt when they are wading along the creek; the impression of her nipple is briefly visible through the wet fabric. When they are captured she is wearing the bikini top again. (01:06:30)
Audio problem: When Bond awakens on the beach and sees Honey Ryder emerging from the water, Honey is singing "Under the Mango Tree", but when she comes out of the water and shakes her head back, her lips are not moving. (01:00:05)
Continuity mistake: As Bond's driver cuts a hard right turn, the tracks from previous takes are on the road. As the pursuing car draws up to that spot, the dust has quickly settled, and the tracks are suddenly gone. (00:19:05)
Visible crew/equipment: As Bond gets into the car in Jamaica, a boom is reflected in the paintwork of the car as he says to take him for a drive. (00:18:30)
Visible crew/equipment: When we see the band playing in Pussfeller's club, the camera shadow can be seen moving over the bassist's jacket. (00:30:40)
Audio problem: Pussfeller says "No-one died from my cooking yet" However the audio is overdubbed as it's too clear. (00:30:20)
Visible crew/equipment: Just as the blind men assassinate Strangways, One looks towards the camera reflecting a very nice stage light in his sunglasses. (00:04:05)
Continuity mistake: When Bond and Honey are looking at the fish for the first time, the position of the fish changes between instant cuts. (01:24:30)
Factual error: When Major Boothroyd gives Bond his new gun, he says that it is a Walther PPK but it actually is a Walther PP. It becomes a PPK in From Russia With Love (1963), the Bond film after this one. (00:13:10)
Factual error: When Bond and Honey are machine-gunned from the boat it is a Bren gun, but the magazine appears to be the wrong way round and faces backwards.
Plot hole: When the receptionist is showing Bond and Honey their rooms she opens the closet with clothes for Honey and says they just got their sizes the night before. How? Dr. No's people didn't know about Honey at all until she showed up with Bond, and they weren't even seen together until they were found in the swamp just several minutes before. (01:19:50)
Audio problem: When the radio controller in London says "Hello, W6N. Report my signals", his lips don't move. (00:05:10)
Visible crew/equipment: When Honey Ryder is telling James that she gets $50 in Miami for her shells, a large boom microphone, grey with silver clips, is visible in the lower left corner of the screen. (01:04:37)
Revealing mistake: When Honey is telling Bond how much money the shells are worth there is someone bobbing up and down just under Honey, possibly a film crew member holding an unseen microphone.
Revealing mistake: Every time a shot of the hallway outside of Bond and Honey's room in Dr. No's lair is shown there are several doors on the same side of the hallway that lead too nowhere. Notice when Bond enters the room looking for Honey near the end we don't see the reverse of the doors from the inside of his room.
Continuity mistake: When we see a close up of Sylvia's £100 tokens, they are mis-aligned and not neat. When the shot changes to the dealer picking them up they are stacked neatly. (00:07:30)
Continuity mistake: When we first see the skyline of London we can see that Big Ben is showing 3 o'clock we also hear Ben's chimes. When we see the casino for the first time the clock is showing 2:50. (00:06:40)