Aunt May: You do too much - college, a job, all this time with me... You're not Superman, you know.
Spidey's organic web shooters are one of the few remaining ideas from an earlier (rejected) script treatment by James Cameron. See more...
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Revealing: When Peter Parker first discovers he can climb up walls (while in citizen clothing), he tests it out by climbing up a building in an alley. During this scene, you can clearly see his clothes hanging out forwards, indicating that the scene was filmed with him crawling along a floor horizontally.
Other: When Jameson is flipping the pictures of Peter's first batch of photographs, you can see (in slow motion or if you have really good vision) that the 3rd picture he looks at changes into an entirely new photograph, when he puts it down. The one he's looking at is Spider-Man on the side of a building, but when it's on the desk it's Spider-Man kicking a thug in the face. The one he then looks at changes too - it was about to have a bit of sky on the left, but then turns into a photo of a street from above, with no sky visible.
Continuity: When Spider-Man saves the baby from the burning building, it's amazing that the baby and the blanket are so clean - no soot, smoke damage, charring, etc. The firefighters have obvious soot around their noses from trying to breathe in that area. Did Spidey manage to give the baby a bath before presenting it to momma?
Continuity: When Spider-Man is chasing the crook with the gun, the crook shoots 7 times through the roof of the car. Shortly afterwards you can see 9 bullet holes in an overhead shot. As the scene moves along there is another overhead shot (just after Spider-Man jumps the bridge) - no bullet holes. Seconds later the bullet holes return.
Continuity: During the World Unity Festival, the Green Goblin seeks revenge upon his ex-coworkers. The Goblin throws a pumpkin bomb at them that makes them all disintegrate and disappear. One of his coworkers had a wheelchair with him, which survives the Goblin's bomb and remains on the balcony. (You can see the wheelchair while they are being bombed and after they disintegrate, all in the same shot.) For the rest of the scene, the wheelchair has disappeared from the balcony.
Continuity: In the final cemetery sequence, Peter and Mary Jane square off for a little heart to heart, with her touching his face tenderly with her black leather gloves. The camera intercuts between front views of both: in hers, her fingers are touching his ear lobe, in his, they are a inch below his ear lobe. In one quick cut of hers, the hand has disappeared completely, then in mid-sentence as they cut back to Peter, it's there again.