The way Blade slices Reinhardt in half is a tribute to the anime film "Vampire Hunter D" in which the main character disposes of numerous enemies (vampire or otherwise) in the exact same manner.

Whenever you see Scud, he's wearing a t-shirt with the logo of the Bureau for Paranormal Research and Defence (B.P.R.D.). The B.P.R.D. is the agency that Hellboy, another comic book character, works for. Hellboy is the next movie to be directed by Guillermo del Toro, Blade II's director.

Michael Jackson was supposed to make a guest appearance in the House Of Pain. If you watch the deleted scenes, Nyssa watches a man empty human entrails from a box in one of the rooms upstairs. This part was supposed to be Jackson, be he had to drop out.

As well as the Scud and del Toro Hellboy connections, Ron Perlman, who plays Reinhardt, plays Hellboy, and Mike Mignola, creator of Hellboy, helped create the look of Blade II as a concept artist. Del Toro and Mignola got together during the making of Blade II to talk about making Hellboy into a movie.

Matt Schulze, the actor who plays Chupa, was actually in Blade 1 as Crease, the vampire who gets his hand chopped off by Blade's sword handle in the Vampire Archives. It was actually his first real movie role.