Continuity mistake: When Khan's number one officer is dying in the arms of Khan, they exchange a word or two before the first officer dies quite dramatically with his eyes open. Khan then fully embraces the corpse and looks up to the viewer screen and vows to get even with Kirk. However, the "corpse", whose eyes are open, closes them upon Khan's embrace.
Plot hole: Why doesn't Reliant know that Khan is exiled here? The Federation is so terrified and opposed to genetic engineering that it's still illegal 300 years after Khan. So why is there no warning along with the data on the Ceti Alpha system? Kirk logged what happened with Khan and his solution of marooning him. Starships use nav data to navigate star systems. Ceti Alpha 6 exploded, yet the helmsman or computer never noticed that there is one less planet than there was when Kirk was there? There is no debris from the explosion? Ceti Alpha 5 is the exact same size and was conveniently blown into the exact same orbit as Ceti Alpha 6 used to have? So there is nothing whatsoever to make the crew even suspect that it's not 6? Enterprise would have to have scanned the planets in the system to know that one was habitable for Khan. Did Ceti Alpha 6's destruction somehow magically turn Ceti Alpha 5 into its exact duplicate? If Starfleet ships have been there to map after Ceti Alpha 6 exploded, none of them bothered to check on the exiles? Pretty callous for Starfleet, don't you think? With the technology and amounts of information available to Starfleet vessels, there is NO logical reason for the Reliant to think that this planet is Ceti Alpha 6. Finally, would the Federation be willing to test a device whose exact effects will be unknown on a planet so close to another inhabited one?
Continuity mistake: Spock's funeral takes place in Torpedo Bay 1, on the starboard side of the ship. The interior shots, however, show Torpedo Bay 2 (you can see the label and the airlock from the beginning of the movie). In addition, the arm that lowers the torpedo is marked with a '2'. Making matters worse, Torpedo Bay 2 was destroyed in the battle with USS Reliant.
Continuity mistake: When Reliant is hit, a large piece of debris falls to the deck, killing Joachim. In the wide shot as Khan makes his way to the debris, a large mass of hanging wires dangles from the ceiling, lower than Khan's head while he is bent over. But the next closer shot has Khan standing erect, lifting the debris, and the wires have vanished. (DVD Director's Cut).
Continuity mistake: When Spock is dying within the glass confines of the ships nuclear power source room with Admiral Kirk (William Shatner) on the opposite side of the glass, in one scene speaking to Spock, Admiral Kirk's red Federation uniform jacket lapel is unbuttoned at the top. In the very next scene the uniform jacket is buttoned.
Audio problem: When Kirk says, "Stop energizers," watch his lips closely - he really says, "Stop engines." Mike Okuda points this out in the text commentary on the Director's Edition DVD. The reason the line (and also the one following it) was redubbed was because someone pointed out during post-production that the engines couldn't simply be stopped on a dime.
Continuity mistake: After the Reliant attack, when Scotty's nephew is talking to Kirk in sickbay, he asks Kirk if the word is given, and Kirk takes his bloody hand then places it against his tunic. After he dies, the next scene shows the bloody handprint in a totally different spot on the tunic.
Continuity mistake: When Kirk and McCoy are in the turbolift, just before Kirk is told about the message from Carol Marcus, they are both standing right next to the door, but in the close up of McCoy, right after Kirk is informed of the message, McCoy has suddenly moved along the wall.
Continuity mistake: When the Enterprise leaves spacedock, Saavik orders 'half impulse'. However, in the exterior shots the impulse engines are not lit. The reason is that ST II uses stock footage from ST I for the spacedock scene. In that movie, the ship leaves the dock using thrusters. The obvious give away is the blue deflector from ST II forward, while ST I had a red deflector. It is a short scene less than 2 seconds, you really have to watch for it. The thrusters are on, as seen in the parts of the scene with the blue deflector, and suddenly they are off and the deflector is red for a few seconds.
Character mistake: While Kahn is "interviewing" Chekhov and Terrell, he stated, "On Earth, two hundred years ago, I was a prince, with power over millions." The official date for this movie is 2285. That would place Kahn on earth around 2085 by this statement. However, it is made clear in the episode "Space Seed" that Kahn and his followers escaped earth in the year 1996: nearly one hundred years earlier. Quite a way off to be a rounding error.
Other mistake: After the Enterprise blows the port nacelle off the Reliant, the destroyed Reliant bridge is shown. The three sheaves of wires hanging from the ceiling should be on a tilt with the angle of the ship rather than straight down. Even accounting for the artificial gravity unit, which could have been damaged.
Continuity mistake: When the Enterprise is making a run for the Mutara Nebula, they show a scene in a photon torpedo bay. On the wall the sign says "TORP BAY 4." The problem is that the Enterprise only has 2 photon torpedo bays. Bay 1 (Starboard side) and Bay 2 (Port side). This version of the Enterprise has no aft photon torpedo bays.
Continuity mistake: When Admiral Kirk and party arrive in the transport pod, they dock on the port side docking port in the secondary hull. But the airlock they emerge from is in the docking port for the port side torpedo bay, as signified by the sign in the background saying "TORP BAY 2." You can see the docking port they are supposed to have docked at to emerge from on the side of the torpedo launchers, just above of the secondary hull. The reason for this is because they re-used the stock footage from ST:TMP when Kirk and Scotty docked at the same docking port.